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Introduction: Ozon is increasingly used for swimming pools disinfecting in Iran. The scope of this cross-sectional study was to the determine ozonation eficiency and influence of pool depth and swimmers gender on the six microbial quality indices. Methods: In this investigation, total Coliform, E.Coli, Heterotrophic bacteria, Streptococcus Feacalis, Staphylococcus Aureus and Pseudomonas Aeroginosa were examined. Sampling was performed six times in every three points (the surface, middle and the end part of the swimming pool), in both men and women' swimming-turn, at the busy time (most costumers' presence) and from the 10-30 centimeters deep in water. Results: The number of Pseudomonas Aeroginosa and Heterotrophic Plate Count were always higher than the Iranian standard level. The number of Staphylococcus Aureus in the all samples of men's turn were lower than the Iranian standard level, but in the women's turn, 10% of the samples were higher than the Iranian standard level. In all the samples, the number of E.Coli and Feacal streptococcus were respectively higher and lower than the Iranian standard level. Conclusion: The observed high pollution levels can be related to inadequate ozone concentration, inappropriate treatment and/or ozone injection points. Although the statistical analysis did not show significant differences between men and women's swimming-turn (a≤ 0/05), there is need for more control in the women's turn because the mean of the most indices in women's turn was higher than that of the men's turn.

Keywords: Swimming Pool, Ozonion, E.Coli, Heterotrophic Bacteria, Feacal Streptococcus, Staphylococcus aerus, Pseudomonas aeruginosa.

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