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Introduction: Many industries need to work all day long and present full-time services. Therefore, individuals and organizations are exposed to new issues like shift work. The necessity of attention to work environment and worker’s health and job stress provoked us to conduct this study to investigate the relationship between job stress and shift work. Methods: In a descriptive and survey study, we investigated job stress of 100 workers of an industry, selected randomly, using job stress and personal-occupational information questionnaires. To test the research hypotheses, we used correlation, t-test and multi-variables regression. Results: The results showed that job stress is higher in shift workers than non-shift workers. Furthermore, a significant relationship was observed between the amount (α=0.01, F=8.99) and source (α=0.05, F=3.76) of stress and demographic variables as well as between the worker’s stress and their level of education. Moreover, this research indicated that it is possible to predict stress from demographic variables (α=0.05, F=0.35) and there is a positive significant correlation between the amount (r=0.78) and source (r=0.54) of stress and shift work. Conclusion: we can conclude that there is a significant relationship between job stress and shift work. So, such studies are suggested the results of which are used to achieve operational solutions and job design interventions related to type of work and ergonomics of work environment to improve worker’s health and job stress.

Keywords: Job stress, Shift work, Dairy industries, Yazd

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