Volume 18, Issue 1 (5-2019)
TB 2019, 18(1): 58-70 |
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Bahador Hajimohammadi 
, Gilda Eslami , Mahsa Aalaei * , Mohammad hasan Ehrampoush , Mohammad ebrahim Rezvani , Hossein Fallahzadeh , Mehrnoosh Shirdeli
, Gilda Eslami , Mahsa Aalaei * , Mohammad hasan Ehrampoush , Mohammad ebrahim Rezvani , Hossein Fallahzadeh , Mehrnoosh Shirdeli
Research Center for Health and Food Safety , aalaeimahsa@gmail.com
Abstract: (2776 Views)
Introduction: Genetically modified plants species of plants are produced by the genetic engineering of agricultural science and the addition of specific genes in their genetic sequences with the aim of optimizing the product and creating desirable traits. This study examined the remains of Cry1A(b) and P35 genes in blood of rats fed with genetically modified rice.
Methods: This study was experimental and interventional and the study population included male and female rats Sprague Dawley (SD), which were divided into two treatment and control groups according to the type of food received. The first group consisted of 50% of the genetically modified rice and second group consisted of 50% of non- genetically modified rice. sampling and DNA extraction from the blood was done after 90 days of feeding the rats with the nutritional pattern using a kit. Quantitative and qualitative study of extracted DNA was performed using agarose gel electrophoresis and spectrophotometry, respectively.
Results: Amplification product with targets P35, Cry1A(b) and T35 was studied using agarose gel electrophoresis 1.5%, which showed that blood samples were negative for the presence of transgenic genes.
Conclusion: The results showed no significant difference in the presence of transgenic genes of p35 and Cry1A(b) in the blood tissue of the treatment and control groups of rats. Therefore, the results of this study reject the possibility of gene transfer to the existing organs of the consumers. Results of this study showed that there is no difference between safety of genetically and non-genetically modified rice from viewpoint of gen transferring.
Methods: This study was experimental and interventional and the study population included male and female rats Sprague Dawley (SD), which were divided into two treatment and control groups according to the type of food received. The first group consisted of 50% of the genetically modified rice and second group consisted of 50% of non- genetically modified rice. sampling and DNA extraction from the blood was done after 90 days of feeding the rats with the nutritional pattern using a kit. Quantitative and qualitative study of extracted DNA was performed using agarose gel electrophoresis and spectrophotometry, respectively.
Results: Amplification product with targets P35, Cry1A(b) and T35 was studied using agarose gel electrophoresis 1.5%, which showed that blood samples were negative for the presence of transgenic genes.
Conclusion: The results showed no significant difference in the presence of transgenic genes of p35 and Cry1A(b) in the blood tissue of the treatment and control groups of rats. Therefore, the results of this study reject the possibility of gene transfer to the existing organs of the consumers. Results of this study showed that there is no difference between safety of genetically and non-genetically modified rice from viewpoint of gen transferring.
Type of Study: Research |
Subject:
Special
Received: 2018/12/12 | Accepted: 2019/02/5 | Published: 2019/05/15
Received: 2018/12/12 | Accepted: 2019/02/5 | Published: 2019/05/15
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