Volume 14, Issue 5 (1-2016)                   TB 2016, 14(5): 173-182 | Back to browse issues page


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Malek M, Labafian Z, hafourzadeh M, Fattahi Bafghi A. Effect of Direct Electricity Current on Viability of Leishmania (L) tropica Promastigotes: an in vitro Study. TB. 2016; 14 (5) :173-182
URL: http://tbj.ssu.ac.ir/article-1-1913-en.html

p.hD Shahid Sadoughi University of Medical Sciences, Yazd, Iran. , afbafghi@ssu.ac.ir
Abstract:   (891 Views)

Introduction: Leishmaniasis is among top ten infectious diseases in the world, including the three diseases is important that the development of vaccines, medicines and insecticides is difficult to control it. The aim of in this study was Effect of direct electricity current on viability of Leishmania (L) tropica promastigotes: an In vitro study.

Methods: Leishmania (L) tropica [MHOM / IR / NADIM3] to an appropriate level in NNN medium and RPMI1640 culture and promastigotes was prepared both stationary and logarithmic phases. The effects of the direct current from the electrodes 2 or 4 cm Leishmania parasite when staining the size of 1 × 1/5 cm in a arose gel and the parasites create both stationary and logarithmic phases were separate. Electrodes are 2 or 4 cm, and when the voltage in the order of 2/5, 3/5 and 4/5 was applied to the electrodes at 5, 10, 15, 20, 25, 30, 35 and 70 minutes.  

Results: Mean number of live promastigotes stationary and logarithmic stages when the distance between the electrodes was 2 cm to voltage 2/5, 3/5 and 4/5 compared with promastigotes in the control group showed significant differences (P=0.003). Also mean number of live promastigotes static stage when the distance between the electrodes was 4 cm to voltage 2/5 and 3/5 V was not significant (P=0.148). And 3/5 and 4/5 volts for voltage are not significant (P>0.05), the mean number of live promastigotes logarithmic stage when the distance between the electrodes was 4 cm, the voltage difference between the number of parasites for 2/5 and 3/5 V  Not significant (P=0.725).

Conclusion: The electrodes are more sensitive and dynamic stage promastigotes lower and higher voltage electric current effect on growth control well.

Full-Text [PDF 583 kb]   (218 Downloads)    
Type of Study: Applicable | Subject: Special
Received: 2016/01/19 | Accepted: 2016/01/19 | Published: 2016/01/19

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